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| Product Name | X-Gal BioChemica |
| Catalog Number | 60365 |
| CAS Number | 7240-90-6 |
| Molecular Weight | 408.33 g/mol |
| Appearance | White to off-white powder |
| Solubility | Soluble in DMSO, DMF, and chloroform |
| Storage Temperature | 2-8°C |
| Purity | ≥ 98% |
| Usage | Substrate for β-galactosidase detection in blue/white screening |
| Application | Used in molecular biology research |
| Alternative Names | 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside |
| Handling | Avoid exposure to light and moisture |
| Shelf Life | 2 years |
FAQ
What is X-Gal BioChemica used for?
X-Gal BioChemica is a chromogenic substrate used in molecular biology for the detection of beta-galactosidase activity. It is widely used in blue-white screening assays to identify recombinant colonies in cloning experiments.
How does X-Gal BioChemica work?
X-Gal BioChemica is cleaved by beta-galactosidase to produce a blue precipitate. Colonies expressing beta-galactosidase will appear blue on agar plates, allowing researchers to easily identify recombinant colonies from non-recombinant colonies.
What are the advantages of using X-Gal BioChemica?
One of the main advantages of using X-Gal BioChemica is its simplicity and convenience in detecting beta-galactosidase activity. It provides a visual marker for recombinant colonies, saving time and labor in screening large numbers of colonies.
Can X-Gal BioChemica be used in combination with other selection methods?
Yes, X-Gal BioChemica is often used in conjunction with other selection markers such as antibiotics. By combining X-Gal BioChemica with antibiotic selection, researchers can have a dual selection system for identifying recombinant colonies.
Is X-Gal BioChemica compatible with different types of agar plates?
Yes, X-Gal BioChemica is compatible with a variety of agar plates commonly used in molecular biology. Researchers can choose the appropriate agar type based on their specific experimental needs.
X-Gal BioChemica is a chromogenic substrate used in molecular biology for the detection of beta-galactosidase activity. It is widely used in blue-white screening assays to identify recombinant colonies in cloning experiments.
How does X-Gal BioChemica work?
X-Gal BioChemica is cleaved by beta-galactosidase to produce a blue precipitate. Colonies expressing beta-galactosidase will appear blue on agar plates, allowing researchers to easily identify recombinant colonies from non-recombinant colonies.
What are the advantages of using X-Gal BioChemica?
One of the main advantages of using X-Gal BioChemica is its simplicity and convenience in detecting beta-galactosidase activity. It provides a visual marker for recombinant colonies, saving time and labor in screening large numbers of colonies.
Can X-Gal BioChemica be used in combination with other selection methods?
Yes, X-Gal BioChemica is often used in conjunction with other selection markers such as antibiotics. By combining X-Gal BioChemica with antibiotic selection, researchers can have a dual selection system for identifying recombinant colonies.
Is X-Gal BioChemica compatible with different types of agar plates?
Yes, X-Gal BioChemica is compatible with a variety of agar plates commonly used in molecular biology. Researchers can choose the appropriate agar type based on their specific experimental needs.
