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Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphatep-Toluidine Salt Solution (50X) [for Western blotting]

    • Product Name Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphatep-Toluidine Salt Solution (50X) [for Western blotting]
    • Alias NBT/BCIP Solution (50X)
    • Einecs 226-255-0
    • Mininmum Order 1 g
    • Factory Site Yudu County, Ganzhou, Jiangxi, China
    • Price Inquiry sales3@ar-reagent.com
    • Manufacturer Nanjing Finechem Holdings Co., Ltd
    • CONTACT NOW
    Specifications

    HS Code

    431523

    Product Name Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
    Abbreviation NBT/BCIP Solution (50X)
    Application Western blotting
    Formulation 50X concentrated solution
    Substrate Type Chromogenic substrate for alkaline phosphatase
    Color Reaction Produces an intense blue-purple precipitate
    Storage Temperature 2-8°C (refrigerated)
    Hazard Classification Harmful if inhaled or swallowed; may cause irritation
    Usage For detection of alkaline phosphatase-conjugated antibodies
    Detection Method Colorimetric/visible color development
    Solubility Ready-to-use aqueous solution
    Component 1 Nitro Blue Tetrazolium (NBT)
    Component 2 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP)
    Dilution Dilute 1:50 with substrate buffer before use
    Light Sensitivity Protect from light

    As an accredited Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphatep-Toluidine Salt Solution (50X) [for Western blotting] factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.

    Packing & Storage
    Packing The solution is packaged in an amber glass bottle, 10 mL volume, with a screw cap and detailed Sigma-Aldrich/Merck label.
    Shipping Shipping for **Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]** (Sigma Aldrich/Merck/TCI) is performed under controlled conditions. The solution is packed to prevent light exposure and temperature fluctuations, typically shipped at 2-8°C, with appropriate hazardous materials labeling, ensuring stability and regulatory compliance.
    Storage Store the Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) at –20°C, protected from light. Keep the container tightly closed in a dry, well-ventilated area, and avoid repeated freeze-thaw cycles. Handle using appropriate protective equipment, as the reagent is light-sensitive and potentially hazardous. Refer to the manufacturer's safety data sheet for detailed handling and disposal instructions.
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