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Product Name: Wright Stain
Synonyms: Romanowsky Stain, Wright’s Blood Stain
Product Use: Diagnostic laboratory reagent for staining blood smears
Manufacturer: Sigma-Aldrich or other chemical suppliers
Emergency Contact: Chemtrec (1-800-424-9300, USA)
Address: Provided by the supplier
GHS Classification: Flammable liquid, Eye irritant, Skin irritant
Signal Word: Danger
Hazard Statements: Highly flammable, may cause serious eye irritation, may cause respiratory irritation
Pictogram: Flame, Exclamation mark
Precautionary Statements: Keep away from heat sources, use protective clothing and eyewear, avoid inhalation, wash hands after handling
Potential Health Effects: Inhalation may cause drowsiness, skin contact may result in redness, ingestion leads to gastrointestinal discomfort
Eosin Y: 1-5%
Methylene Blue: 1-5%
Sodium Phosphate Dibasic: 1-5%
Methanol: 80-90%
Giemsa Solution Additives: May contain glycerol, acetone, Azure B, supplemental dyes based on manufacturer
CAS Numbers: Eosin Y: 17372-87-1; Methylene Blue: 61-73-4; Methanol: 67-56-1; Sodium Phosphate Dibasic: 7558-79-4
Inhalation: Move to fresh air. Restore breathing if stopped. Seek medical attention for persistent symptoms.
Skin contact: Remove contaminated clothing. Wash affected skin with soap and running water for at least 15 minutes.
Eye contact: Rinse immediately with water for 15 minutes, lifting eyelids to flush thoroughly. Seek medical attention.
Ingestion: Do not induce vomiting. Rinse mouth with water. Seek immediate medical advice.
Suitable Extinguishing Media: Alcohol-resistant foam, carbon dioxide, dry chemical powder
Unsuitable Extinguishing Media: Strong water jets
Specific Hazards: Vapors may form explosive mixtures with air; combustion produces toxic fumes (carbon monoxide, formaldehyde)
Protective Equipment: Wear self-contained breathing apparatus, full firefighting gear
Advice for Firefighters: Keep containers cool with water spray. Prevent runoff from entering drains.
Personal Precautions: Evacuate area and ventilate. Use protective gloves, eyewear, and mask.
Environmental Precautions: Prevent spillage from seeping into drains, soil, waterways.
Spill Clean-up Methods: Absorb with non-combustible, inert material (sand, earth, vermiculite). Scoop into container for disposal. Wash spill site with water and detergent after material pick-up.
Special Considerations: Alert emergency services for large spills; use spark-proof tools
Handling: Avoid inhalation of vapors, contact with eyes and skin. Only open containers in well-ventilated areas. No smoking or open flames.
Storage: Store in a cool, dry, well-ventilated place away from oxidizing agents, direct sunlight, and sources of heat. Keep container tightly sealed when not in use.
Incompatibilities: Strong oxidizers, acids, alkalis.
Special Storage Conditions: Maintain at temperatures between 15°C and 30°C. Prevent freezing.
General Hygiene: Wash thoroughly after handling. Do not eat, drink, or smoke near chemical.
Exposure Limits: Methanol: OSHA PEL 200 ppm (260 mg/m³), ACGIH TLV 200 ppm
Engineering Controls: Use chemical fume hood or local exhaust.
Personal Protection: Wear chemical splash goggles, lab coat, nitrile gloves.
Respiratory Protection: Use NIOSH-approved respirator if ventilation is inadequate.
Environmental Controls: Prevent leakage to drains, surface and groundwater.
General Recommendations: Remove contaminated clothing immediately; wash hands thoroughly after use.
Appearance: Dark blue to violet liquid
Odor: Alcohol-like
pH: 6.5 – 7.5
Boiling Point: Approx. 64°C (Methanol component)
Melting Point: < –85°C (Methanol component)
Flash Point: 12°C (closed cup, Methanol)
Vapor Pressure: 127 mmHg at 20°C (Methanol)
Solubility: Completely soluble in water and methanol
Density: Approx. 0.79 g/cm³ (Methanol basis)
Evaporation Rate: Rapid
Partition Coefficient: Not established for whole mixture
Auto-ignition Temperature: 464°C (Methanol)
Chemical Stability: Stable under normal storage and use conditions.
Reactivity: Reacts with strong oxidizers, acids, alkalis.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, formaldehyde, nitrogen oxides, toxic dye byproducts.
Conditions to Avoid: Heat, sparks, open flames, direct sunlight.
Polymerization: Will not occur under recommended conditions.
Incompatibilities: Mercury salts, hydrogen peroxide, perchlorates.
Acute Toxicity: Methanol is toxic if swallowed, inhaled, or absorbed through skin; LD50 (oral, rat): 5628 mg/kg (methanol); Eosin and methylene blue toxicity is low but may cause local irritation.
Chronic Toxicity: Methanol: cumulative central nervous system depression, may cause visual impairment and organ damage with prolonged exposure.
Routes of Exposure: Inhalation, ingestion, skin, and eye contact.
Carcinogenicity: Not classified as human carcinogen
Symptoms: Eye and respiratory tract irritation, headache, dizziness, nausea, blurred vision
Sensitization: No known sensitization data
Other Effects: Prolonged skin contact may lead to dermatitis or drying.
Toxicity: Methanol is classified as moderately toxic to aquatic life. Dyes may persist in environment and stain biological tissue.
Mobility: Methanol is highly mobile in soil and water due to solubility.
Persistence and Degradability: Methanol readily biodegradable; Eosin and methylene blue may persist.
Bioaccumulative Potential: Low for methanol; colored components not expected to bioaccumulate significantly.
Other Adverse Effects: Large spills may affect oxygen transfer in water, disrupt microbial populations.
Environmental Precautions: Do not allow substantial release into sewers or waterways.
Disposal Method: Dispose of chemical waste by licensed hazardous waste contractor in accordance with federal, state, and local regulations.
Contaminated Packaging: Triple rinse containers before disposal, puncture and send to approved landfill or incinerator.
Special Considerations: Do not re-use containers. Never dispose down drain or natural environments.
Waste Codes: Methanol: D001 (flammable), others as appropriate for locale.
UN Number: UN1230 (Methanol solution)
Proper Shipping Name: Methanol Solution
Hazard Class: 3 (Flammable Liquid)
Packing Group: II
Labels: Flammable Liquid
Marine Pollutant: Not listed as marine pollutant (check local authority requirements)
Special Precautions: Keep away from food and feed. Transport in upright sealed containers.
OSHA Hazard Communication: Meets the criteria.
TSCA Inventory: All components listed or exempted.
SARA 302/313: Methanol subject to notification.
California Proposition 65: Methanol is listed.
WHMIS (Canada): B2 (Flammable Liquid), D2B (Toxic material)
EU Regulation: Methanol classified as Category 2 reproductive toxin, Risk Phrases R11, R23/24/25, R39/23/24/25
Other Regulations: Comply with local, state, national, and international laws regarding chemical safety.
