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Product Name: Trypsin-EDTA Solution, 0.25%, 10X
Synonyms: Trypsin-EDTA, Trypsin solution
Chemical Formula: Not applicable (mixture of trypsin enzyme and EDTA in aqueous buffer)
Manufacturer: Refer to supplier's contact details
Intended Use: Cell culture applications such as detachment and dissociation of adherent cells
Contact Information: Emergency telephone number provided by supplier, non-emergency questions route through procurement or laboratory supervisor
CAS Numbers: Trypsin: 9002-07-7; EDTA: 60-00-4
GHS Classification: Irritant to eyes and skin, low inhalation risk at normal use; potential allergen
GHS Label Elements: Exclamation mark for skin and eye irritation, advisory for respiratory sensitization
Signal Word: Warning
Hazard Statements: Causes serious eye irritation, may cause mild skin irritation, risk of allergic reaction in susceptible individuals
Precautionary Statements: Avoid contact with eyes, skin, or clothing. Wear protective equipment as required.
Target Organs: Eyes, respiratory system, skin
Pictograms: Exclamation mark
Trypsin: 0.25% w/v, CAS 9002-07-7, RTECS YD6476000
EDTA (Ethylenediaminetetraacetic acid): 0.02-0.5% w/v, CAS 60-00-4
Sodium Chloride: 0.85-1% w/v, CAS 7647-14-5
Aqueous buffer: Typical content; proprietary or trade-secret
Other Ingredients: Water for injection or cell-culture grade water. Sterility ensured by filtration.
Eye Contact: Immediately flush with plenty of water for a minimum of 15 minutes, removing contact lenses if present, seek medical attention
Skin Contact: Wash thoroughly with soap and water, remove contaminated clothing, consult a physician if symptoms persist
Inhalation: Move affected person to fresh air, keep warm, seek medical advice if irritation or sensitization develops
Ingestion: Rinse mouth with water, do not induce vomiting unless instructed by medical personnel, seek medical advice
Notes for Physician: Symptomatic treatment only, monitor for respiratory distress or allergic reaction
Suitable Extinguishing Media: Use foam, CO2, dry chemical powder, or water mist for fires in surrounding area
Unsuitable Extinguishing Media: Strong water jets may spread spilled liquid
Specific Hazards: Thermal decomposition may generate small amounts of carbon oxides, nitrogen oxides, and ammonia
Protective Equipment: Firefighters should wear self-contained breathing apparatus and full protective gear
Advice for Firefighters: Prevent contaminated extinguishing water from entering drains or watercourses
Personal Precautions: Wear laboratory gloves, eye and face protection, lab coat. Avoid inhaling aerosols.
Environmental Precautions: Prevent spillage from reaching drains, surface waters, or soil
Containment: Absorb with inert material (e.g., paper towels, vermiculite), place in chemical waste container
Clean-Up Methods: Clean affected area with water and detergent afterwards, ventilate area, dispose of material as per regulatory requirements
Handling Practices: Use in well-ventilated areas, avoid splashing and aerosols, wear protective gloves and eyewear, refrain from eating, drinking or smoking in work area
Storage Conditions: Store at 2-8°C, protected from light, keep container tightly closed and labeled
Storage Incompatibilities: Avoid strong acids and bases, keep away from incompatible chemical substances
Transfer Precautions: Use secondary containment where feasible
Occupational Exposure Limits: Not established for trypsin or EDTA; minimize aerosol generation
Engineering Controls: Use fume hood or biosafety cabinet during procedures that might generate mist, provide eyewash stations
Personal Protection - Eyes/Face: Safety goggles or face shield
Personal Protection - Hands: Disposable nitrile or latex gloves
Personal Protection - Body: Laboratory coat
Respiratory Protection: Not normally required, but masks may be worn if sensitive or at risk of aerosol exposure
Appearance: Clear or slightly yellow liquid
Odor: Slight proteinaceous odor
pH: 7.2 - 7.6 (typical)
Melting/Freezing Point: Approximately 0°C (water-based solution)
Boiling Point: Near 100°C
Flash Point: Not applicable (aqueous solution)
Evaporation Rate: Similar to water
Solubility: Completely miscible in water
Relative Density: Near 1.0 g/cm³
Partition Coefficient: Not determined
Decomposition Temperature: Above 60°C (loss of enzyme activity)
Chemical Stability: Stable under recommended storage conditions; loss of activity at elevated temperature or extended exposure to light
Reactivity: No dangerous reactions known under normal laboratory conditions
Conditions to Avoid: Prolonged exposure to heat, strong acids or bases, sunlight
Incompatible Materials: Strong oxidizing agents, acids, bases
Hazardous Decomposition Products: Carbon oxides, nitrogen oxides, small peptides on digestion
Acute Toxicity: Trypsin and EDTA solutions are low hazard at dilution used; higher concentration may cause tissue damage
Routes of Exposure: Eyes, skin, inhalation of mist, ingestion
Symptoms: Redness, irritation, tearing (eyes); rash or irritation (skin); respiratory discomfort if inhaled
Sensitization: Possible for sensitive individuals—some reports of allergic reactions
Chronic Effects: Repeated contact may cause dermatitis or exacerbation of pre-existing allergies
Mutagenicity/Carcinogenicity: No data indicating carcinogenic risk in humans at these concentrations
LD50: Not established for mixture; components generally low in acute toxicity at laboratory concentrations
Ecotoxicity: Low predicted hazard due to rapid dilution and biodegradation; large spills may interfere with aquatic organisms by enzyme activity
Persistence and Degradability: Readily bio-degradable protein, EDTA may persist in environment but not at low concentrations used
Bioaccumulative Potential: Low for both trypsin and EDTA in dilute solution
Mobility in Soil: High (water soluble); rapid dispersion
Aquatic Toxicity: No chronic effects at laboratory spill levels; avoid release to sewers or waterways
Waste Treatment Methods: Follow institutional and local regulations; typically, deactivate with excess water, then dispose as non-hazardous biological waste
Container Disposal: Triple-rinse bottles and containers, dispose as laboratory plastic waste or glass waste as appropriate
Precautions: Avoid disposal into the environment, do not re-use containers
Regulatory Status: Not considered hazardous waste at this dilution; confirm local, state, or provincial rules
UN Number: None assigned for this mixture
UN Proper Shipping Name: Not regulated as dangerous goods
Transport Hazard Class: Not classified
Packing Group: Not applicable
Special Precautions for Transport: Keep containers upright and secure, protect from direct sunlight and freezing
Environmental Hazards: Not a marine pollutant
OSHA Status: Not regulated as a hazardous substance
TSCA Status: Ingredients listed or exempt
DSL/NDSL (Canada): Components listed
WHMIS Classification: Not a controlled product under WHMIS
EU Regulations: Not classified as hazardous under Regulation (EC) No 1272/2008 (CLP)
SARA Title III: Not listed
California Prop 65: No components known to cause cancer or reproductive toxicity
Other National Regulations: Ensure compliance with regional and institutional biosafety standards
