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Product Name: Trypan Blue
Chemical Formula: C34H28N6O14S4
CAS Number: 72-57-1
Manufacturer: Identified by supplier invoice
Recommended Use: Biological staining, cell viability
Contact Information: Detailed on shipment paperwork
Synonyms: Direct Blue 14, Diamine Blue
Emergency Phone: Local poison control or emergency number
Relevant Identified Uses: Laboratory research, microscopy staining
GHS Classification: Eye Irritation (Category 2), Acute Oral Toxicity (Category 4), Skin Irritation (Category 2)
Signal Word: Warning
Pictograms: Exclamation Mark
Hazard Statements: Causes serious eye irritation, may cause skin irritation, can be harmful if swallowed
Precautionary Statements: Avoid contact with skin and eyes, do not breathe dust, wash hands thoroughly after handling
Potential Health Effects: Inhalation may cause respiratory tract irritation, ingestion could result in nausea, abdominal pain, blue discoloration of mouth and mucous membranes; exposure to skin might lead to redness or rash
Chemical Name: Trypan Blue
CAS Number: 72-57-1
EC Number: 200-786-7
Weight %: >99% pure
Impurities: No significant hazardous impurities detected as per supplier’s certificate of analysis
Eye Contact: Rinse gently with water for at least 15 minutes, lift eyelids, seek medical advice if irritation persists
Skin Contact: Remove contaminated clothing, wash promptly with soap and plenty of water
Inhalation: Move person to fresh air, keep at rest, seek medical support if symptoms develop
Ingestion: Rinse mouth with water, avoid inducing vomiting, seek immediate medical attention
Symptoms/Effects: Redness, rash, temporary blue discoloration of tissues, gastrointestinal discomfort if ingested
Suitable Extinguishing Media: Use carbon dioxide, dry chemical powder, alcohol-resistant foam, or water spray
Specific Hazards: May emit toxic fumes of nitrogen oxides, sulfur oxides, and carbon oxides under fire conditions
Protective Equipment: Firefighters require self-contained breathing apparatus, chemical-resistant clothing
Advice for Firefighters: Move containers from risk area, avoid breathing smoke, cool containers with flood water if safe
Personal Precautions: Use personal protective gear, ensure adequate ventilation, avoid dust formation
Environmental Precautions: Prevent from entering surface water or sewer, contain spill with inert absorbent
Spill Clean-up Methods: Sweep up and shovel into suitable containers for disposal, clean affected area with suitable detergent, collect all residues thoroughly
Handling: Wear laboratory gloves, eye protection, and laboratory coat, work in a fume hood if dust risk exists, use proper laboratory practices
Storage: Store tightly capped at room temperature, keep dry in a well-ventilated area, avoid direct sunlight, separate from strong oxidizing agents
Occupational Exposure Limits: No specific exposure limits set by OSHA, NIOSH, or ACGIH
Engineering Controls: Use with exhaust ventilation, safety showers, eyewash stations
Personal Protective Equipment: Gloves (nitrile or latex), safety glasses or goggles, laboratory coat, respiratory protection in dusty environments
Hygiene Measures: Do not eat, drink, or smoke while handling, wash hands thoroughly after use, remove contaminated clothing promptly
Appearance: Dark blue crystalline powder
Odor: Odorless
Odor Threshold: Not determined
pH (1% solution): Approximately 6-7
Melting Point: Decomposes above 300°C
Boiling Point: Not applicable
Solubility: Soluble in water, yielding blue solution
Vapor Pressure: Not relevant at room temperature
Density: Approximately 1.4 g/cm³
Partition Coefficient (n-octanol/water): Not established
Decomposition Temperature: Above 300°C
Flash Point: Not flammable under typical conditions of use
Chemical Stability: Chemically stable under recommended storage conditions
Incompatible Materials: Strong oxidizers, strong acids
Hazardous Decomposition Products: Carbon oxides, nitrogen oxides, sulfur oxides on thermal decomposition
Polymerization: Does not polymerize under normal circumstances
Conditions to Avoid: Heating to high temperatures, contact with strong oxidizing agents
Routes of Exposure: Inhalation, ingestion, skin and eye contact
Acute Toxicity: Oral LD50 in rats: > 500 mg/kg
Chronic Effects: Prolonged exposure may affect blood chemistry, kidney, and liver function in animal studies
Skin Corrosion/Irritation: May cause dryness, redness
Serious Eye Damage/Irritation: Causes eye irritation
Carcinogenicity: IARC, NTP, OSHA do not classify Trypan Blue as carcinogenic, though animal studies suggest potential risks from chronic high-level exposure
Reproductive Toxicity: No conclusive human data available
Other Effects: Temporary tissue discoloration, possible allergic reactions
Aquatic Toxicity: Harmful to aquatic organisms based on available animal data
Persistence and Degradability: Not rapidly biodegradable under typical environmental conditions
Bioaccumulative Potential: Limited bioaccumulation expected due to physical-chemical properties
Mobility in Soil: Moderate; soluble in water, may migrate into aquatic environments
Other Adverse Effects: Spills can cause blue staining of water and soil, potentially affecting local ecology by coloration and chemical stress
Disposal Methods: Dispose of contents in accordance with local, regional, and national environmental regulations, preferable via authorized chemical waste collection
Contaminated Packaging: Rinse thoroughly before reuse or disposal, manage as hazardous laboratory waste
Precautions: Prevent substance from entering natural water bodies, do not dispose of in municipal waste streams
UN Number: Not classified as hazardous for transport
Proper Shipping Name: Trypan Blue
Transport Hazard Class: Not regulated
Packing Group: Not assigned
Environmental Hazards: Limited evidence for acute transport-related risk; contain all spills during transport
Special Precautions: Use clearly labeled containers, avoid direct exposure during transit
US TSCA: Listed
EU REACH: Meets requirements for research chemical registration
OSHA: Not listed as hazardous under 29 CFR 1910.1200
SARA Title III: Not reportable
DSL/NDSL (Canada): Listed
Other Regulatory Info: Handle according to Good Laboratory Practice and institutional policies
Labeling Requirements: Detailed chemical name, hazard pictogram, warning statements per GHS
