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Product Name: Resazurin Sodium Salt
Chemical Formula: C12H6NNaO4
Chemical Name: 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
Synonyms: Resazurin sodium, Alamar Blue, Diazo-resorcinol sodium salt
CAS Number: 62758-13-8
EC Number: 263-467-1
Recommended Use: Cell viability assays, metabolic indicator
Manufacturer/Supplier: Chemical and laboratory suppliers worldwide
Contact Information: Refer to the supplier distributor’s safety office or local representative
Emergency Phone: Information found on supplier web pages or local poison control centers
Classification: Not classified as hazardous according to GHS guidelines under normal laboratory use
Label Elements: Non-hazardous, no pictograms required
Hazard Statements: Dust may irritate respiratory system or eyes.
Precautionary Statements: Avoid inhaling dust, contact with skin, and exposure to eyes. Use sensible lab safety habits.
Potential Health Effects: May irritate the skin, eyes, or be harmful if swallowed in quantity. Direct inhalation can bother sensitive individuals. Susceptible users should avoid ingestion or inhalation of powder.
Environmental Hazards: Not considered environmentally hazardous in small quantities but large quantities may disrupt microbial function in waterways. Avoid disposing significant amounts in drains.
Chemical Identity: Resazurin Sodium Salt
Purity: Generally >95% pure in research grade product
Impurities: May contain trace solvents or starting material residues from synthesis
Other Components: None typically listed beyond color stabilizers in some suppliers’ batches
CAS Number: 62758-13-8
Inhalation: Remove exposed person to fresh air. If trouble breathing, seek medical attention. Most reactions are limited to mild irritation.
Skin Contact: Immediately remove contaminated clothing. Wash skin gently with lots of soap and water. If irritation persists, consult a healthcare provider.
Eye Contact: Rinse eyes carefully with water for at least 15 minutes lifting upper and lower lids occasionally. Look up local medical advice if any symptoms continue.
Ingestion: Rinse mouth thoroughly. Do not induce vomiting. Drink water if conscious. Get medical help for large amounts or if symptoms develop.
Medical Attention: None expected to be required for minor exposure; provide symptomatic care if reaction occurs.
Suitable Extinguishing Media: Use water spray, carbon dioxide, dry chemical powder, or foam. Select media suitable for surrounding material fire.
Special Hazards: Product burns with possible release of carbon oxides, nitrogen oxides, and sodium oxides.
Firefighter Protection: Wear self-contained breathing apparatus and full protective gear. Avoid inhaling decomposition products.
Thermal Decomposition: Emission of toxic fumes is possible.
Advice: Control run-off water from containment to prevent entry into drains.
Personal Precautions: Wear gloves, protective clothing, and safety glasses to prevent direct contact.
Environmental Precautions: Prevent large spills from entering sewers, soil, or watercourses.
Spill Cleanup Methods: Sweep or scoop material up, avoiding dust formation. Dispose of cleanup materials in chemical waste containers. Wipe area with wet towel or mop after dry collection.
Other Notes: Wash hands thoroughly after cleanup. Notify supervisor in laboratory settings.
Handling: Avoid dust creation. Use fume hood or well-ventilated area. Practice good hygiene. Don’t eat, drink, or smoke when using this chemical. Keep container tightly closed.
Storage: Store in cool, dry, dark place away from incompatible substances such as oxidizers or acids. Use airtight, labeled containers. Avoid moisture and high humidity as product can decompose.
Incompatibles: Strong acids, strong oxidizing agents, reducing agents. Do not store with food or animal feed.
Conditions to Avoid: Excess heat, ignition sources, direct sunlight, open air storage, and sources of contamination.
Engineering Controls: Employ chemical fume hood, local exhaust, or general ventilation to minimize airborne dust.
Respiratory Protection: Use suitable NIOSH or equivalent certified dust mask if powder is accessible or if dust generation is unavoidable.
Skin Protection: Wear chemical-resistant gloves and laboratory coat.
Eye Protection: Lab goggles or face shield required in splatter risk environments.
Hygiene Measures: Wash thoroughly after handling, especially before eating or drinking. Remove contaminated clothing and wash before reuse.
Exposure Limit Values: Not established for resazurin sodium salt; maintain airborne dust levels as low as possible.
Appearance: Dark blue to purple powder or crystalline solid
Odor: Odorless or faintly chemical
Molecular Weight: 251.17 g/mol
Solubility: Highly soluble in water, producing a blue solution
pH (1% Solution): About 6.0 to 7.5 depending on purity and source
Melting Point: Decomposes above 250°C
Boiling Point: Not applicable, decomposes
Vapor Pressure: Negligible at ambient conditions
Bulk Density: Variable depending on grind
Flash Point: Not flammable under standard conditions
Partition Coefficient: Not determined for sodium salt
Other Data: May stain surfaces and skin; handle carefully to avoid contamination.
Chemical Stability: Stable under recommended storage conditions. Decomposes under exposure to light or moisture over time.
Reactivity: Not reactive under normal storage and use.
Possible Hazardous Reactions: Contact with strong oxidizers or strong acids can cause decomposition.
Decomposition Products: Produces carbon oxides, nitrogen oxides, sodium compounds when burned or degraded.
Conditions to Avoid: Light, heat, humidity, contact with acid/oxidizer chemicals.
Acute Toxicity: Oral or dermal toxicity expected to be low based on available data. Literature does not cite significant acute toxicity at common laboratory exposures.
Chronic Exposure: No long-term studies showing chronic toxicity in humans; avoid chronic inhalation and skin contact.
Routes of Exposure: Inhalation, ingestion, skin, and eye contact.
Symptoms of Exposure: Mild skin or respiratory irritation, possible eye irritation, staining.
Carcinogenicity: No evidence for carcinogenicity in animals or humans.
Mutagenicity: Not known to be mutagenic.
Other Data: Handle with appropriate care, as with all laboratory materials whose systemic effects may not be fully characterized.
Eco-toxicity: Not classified as environmentally hazardous in small quantities. In larger releases or persistent use, may harm microorganisms and basic aquatic life.
Mobility: Soluble in water; can migrate through soil and surface water if spilled.
Persistence/Degradability: Expected to degrade slowly in water and soil, especially under light exposure.
Bioaccumulation Potential: Low; not expected to accumulate in animal tissues.
Aquatic Toxicity: Very little evidence of acute aquatic toxicity at laboratory concentrations.
Precautions: Avoid dumping large volumes into water systems.
Disposal Method: Place material and contaminated packaging in appropriate chemical waste containers. Dispose of through licensed chemical waste service.
Preventative Practices: Dilute small residues with water before disposal if permitted. Do not flush concentrated product to wastewater systems.
Regulations: Comply with local, regional, and national environmental regulations for chemical waste.
Container Handling: Triple rinse or decontaminate containers before disposal. Remove hazardous labels before recycling.
UN Number: Not classified under any UN transport regulation.
UN Proper Shipping Name: Not regulated.
Transport Hazard Class: None.
Packing Group: None.
Environmental Hazards During Transport: Not expected to pose environmental risk.
Special Precautions: Protect from moisture, shocks, direct sunlight.
Transport Regulations: Follow general chemical transport safety and regional guidance.
Safety, Health, Environment Regulations: Not subject to special registration requirements in the United States, European Union, or many national agencies for research use.
OSHA: Not classified as hazardous under OSHA regulations.
TSCA: Listed, permitted for research and development.
EU Regulations: Not subject to classification or labeling as hazardous; available on REACH inventory.
Canadian WHMIS: Not controlled.
Other Country Regulations: Check specific national health and chemical agency databases for up-to-date registration or prohibition info.
Other Considerations: Follow institutional requirements and national lab safety guidelines for storage and use.
