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Material Safety Data Sheet for Red Blood Cell Lysing Buffer

Identification

Product Name: Red Blood Cell Lysing Buffer
Common Use: Blood cell lysis for laboratory analysis, hematology testing
Manufacturer: [Insert company name]
Address: [Insert full address]
Telephone: [Insert phone number]
Emergency Contact: [Insert emergency contact details]
Recommended Use: Suitable for in vitro diagnostic procedures in clinical settings; not designed for therapeutic or in vivo use

Hazard Identification

GHS Classification: Eye Irritant (Category 2), Skin Irritant (Category 2)
Signal Word: Warning
Hazard Statements: Causes serious eye irritation, may cause skin irritation, harmful if swallowed
Precautionary Statements: Wear protective gloves, avoid inhalation of vapors or mists, wash hands thoroughly after handling, keep container tightly closed

Composition / Information on Ingredients

Ammonium Chloride: CAS No. 12125-02-9, typically 1–2%
Potassium Bicarbonate: CAS No. 298-14-6, typically 1–1.5%
EDTA (Ethylenediaminetetraacetic acid, disodium salt): CAS No. 6381-92-6, typically 0.1–0.2%
Water, Deionized: CAS No. 7732-18-5, balance to 100%

First Aid Measures

Inhalation: Move person to fresh air, keep comfortable for breathing, seek medical attention if symptoms persist
Skin Contact: Remove contaminated clothing, rinse skin under running water for several minutes, seek medical advice if irritation continues
Eye Contact: Rinse cautiously with water for at least 10 minutes, keep eyelids open and away from eye, remove contact lenses if present and easy to do, seek medical attention immediately
Ingestion: Do not induce vomiting, rinse mouth thoroughly, drink water if person is alert, get medical help right away

Fire-Fighting Measures

Suitable Extinguishing Media: Use water spray, foam, dry chemical, or carbon dioxide
Specific Hazards: In case of fire, decomposition may release toxic fumes such as ammonia, nitrogen oxides, chlorine-containing gases
Protective Equipment: Firefighters require self-contained breathing apparatus and full protective clothing

Accidental Release Measures

Personal Precautions: Avoid eye and skin contact, ventilate area, put on protective gloves and goggles
Spill Cleanup: Absorb with inert material (e.g., dry sand, earth), place in chemical waste container, wash spill area with copious amounts of water
Environmental Precautions: Prevent entry into drains, surface water, or ground water

Handling and Storage

Handling: Wear personal protective equipment, use inside chemical fume hood or well-ventilated area, avoid inhalation, avoid contact with eyes and skin, do not eat, drink or smoke during use
Storage: Store in closed, appropriately labeled containers, keep at room temperature out of direct sunlight, separate from strong acids or oxidizers, keep away from children and unauthorized personnel

Exposure Controls and Personal Protection

Engineering Controls: Use local exhaust or general laboratory ventilation to control exposure
Personal Protective Equipment: Safety goggles, laboratory coat, nitrile or latex gloves, face shield if risk of splashing exists
Occupational Exposure Limit: Ammonium chloride TLV: 10 mg/m³ (ACGIH), no specific limits for other ingredients
Hygiene Measures: Wash hands after handling, do not touch face or eyes with contaminated gloves or hands, remove contaminated clothing promptly

Physical and Chemical Properties

Appearance: Clear, colorless liquid
Odor: Slightly ammoniacal
pH: Typically 7.2–7.6
Melting/Freezing Point: ~0°C (water-based)
Boiling Point: ~100°C
Flash Point: Not flammable
Solubility: Fully miscible in water
Vapor Pressure: As for water
Density: Close to 1 g/cm³ at 20°C

Stability and Reactivity

Chemical Stability: Stable under recommended storage conditions
Reactivity: Reacts with strong acids to release toxic gases (e.g., chlorine, ammonia), avoid contact with oxidizers
Hazardous Decomposition: Ammonia, nitrogen oxides, chlorine compounds possible in fire or high heat

Toxicological Information

Acute Toxicity: Harmful if swallowed in large quantities, may irritate mucous membranes, respiratory tract, skin, and eyes
Chronic Effects: Prolonged exposure may cause skin and eye irritation, not classified as carcinogenic, mutagenic, or teratogenic by IARC, NTP, or OSHA
Symptoms of Overexposure: Burning sensation in eyes, coughing, skin redness, headache, nausea possible with significant exposure

Ecological Information

Ecotoxicity: Solutions containing ammonium chloride and EDTA can harm aquatic life in high concentrations
Persistence and Degradability: Components break down slowly in environment, EDTA is not rapidly biodegradable
Mobility in Soil: High mobility expected due to water solubility
Bioaccumulation Potential: Low for most ingredients in working concentrations
Avoid Environmental Release: Dilute solutions should be disposed of properly to avoid environmental harm

Disposal Considerations

Disposal Methods: Do not pour down drain or release untreated into environment, collect waste in clearly labeled chemical containers
Regulatory Disposal: Hand over chemical waste to authorized hazardous waste disposal facility in accordance with local, regional, and national regulations
Contaminated Packaging: Dispose of as hazardous solid waste, rinse thoroughly if recycling or reusing container

Transport Information

UN Number: Not regulated for transport under most conditions due to dilute formulation
Proper Shipping Name: Not required
Transport Hazard Class: Not classified
Packing Group: Not assigned
Special Transport Precautions: Carry in upright containers, avoid leakage, shield from physical damage during transit

Regulatory Information

OSHA Status: Components present at concentrations below thresholds for classification as hazardous
SARA Title III: Ammonium chloride subject to reporting, no extremely hazardous substances present
TSCA Inventory: All chemical substances listed
WHMIS (Canada): Classified as Class D2B – Toxic material causing other toxic effects
Labeling Requirements: GHS-compliant hazard label, eye and skin irritant icons, precautionary statements