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Material Safety Data Sheet – NUTRIENT MIXTURE F-10 HAM

Identification

Product Name: Nutrient Mixture F-10 Ham
Chemical Family: Growth media
Product Use: Cell culture
Manufacturer: [Manufacturer info, typically found on container]
Emergency Contact: [24-hour emergency number, as shown on label]
Date Prepared: [Date from supplier]
Synonyms: F-10, Ham’s F-10 nutrient mix
Catalogue Number: [Specific catalogue ID, usually on bottle]
Recommended Restriction: For laboratory use only, not for drug, food, or household use

Hazard Identification

Classification: Not classified as hazardous according to GHS (Globally Harmonized System)
Hazard Statements: May cause mild eye, skin, and respiratory tract irritation
Pictograms: None assigned
Signal Word: None required
Precautionary Statements: Avoid contact with eyes and prolonged skin exposure, wash thoroughly after handling
Other Hazards: Dust may form explosive mixture with air under rare conditions; keep container closed
Medical Conditions Aggravated: Individuals with pre-existing allergies or compromised respiratory systems may notice irritation more acutely

Composition / Information on Ingredients

Chemical Blend: Contains inorganic salts, amino acids, vitamins, and glucose
Major Components: Sodium chloride, potassium chloride, calcium chloride, magnesium sulfate, sodium phosphate, L-glutamine, glucose, sodium bicarbonate, ferric nitrate, amino acids (including L-arginine, L-asparagine, glycine, L-histidine), vitamins (including biotin, folic acid, riboflavin, thiamine), phenol red (optional, coloring agent)
CAS Numbers: Mixture does not have one; individual CAS numbers available for each component
Concentration Range: Each individual ingredient at percent by weight varies by formulation; not all ingredients present in reportable amounts

First Aid Measures

General Advice: Move victim to fresh air if inhaled; rinse mouth if swallowed accidentally; seek medical help if symptoms persist
Eye Contact: Flush eyes with plenty of water for at least 15 minutes, remove contact lenses if possible
Skin Contact: Wash with soap and water, remove contaminated clothing
Inhalation: Remove to fresh air, support breathing
Ingestion: Rinse mouth, do not induce vomiting unless advised by medical professional

Fire-Fighting Measures

Suitable Extinguishing Media: Use water spray, foam, dry chemical, or carbon dioxide
Unsuitable Extinguishing Media: High-pressure water streams
Unusual Fire and Explosion Hazards: Fine dust may present minimal explosion hazard in confined spaces
Special Protective Equipment: Firefighters should wear self-contained breathing apparatus and complete protective gear
Combustion Products: May release toxic vapors such as carbon oxides and nitrogen oxides during fire

Accidental Release Measures

Personal Precautions: Use protective gloves, eye protection, face protection
Environmental Precautions: Avoid discharge into drains or natural waterways
Cleanup Methods: Sweep or scoop spilled material into suitable container; avoid creating dust; ventilate area well
Decontamination: Wash the affected area with water after cleanup
Waste Disposal: Dispose in accordance with local, regional, or national regulations

Handling and Storage

Precautions for Safe Handling: Avoid breathing dust; handle in well-ventilated areas; prevent skin and eye contact
Storage Conditions: Keep container tightly closed and dry; store at recommended temperature provided on label; avoid humidity or excessive heat
Separation from Incompatibles: Do not store with strong acids or bases; separate from oxidizers
Other Guidelines: Use clean equipment to prevent contamination; label all containers clearly
Hygiene Practices: Wash hands before breaks and at end of procedure; avoid eating, drinking, or smoking in lab area

Exposure Controls and Personal Protection

Engineering Controls: Use local exhaust or general ventilation to keep airborne levels low
Personal Protection: Wear chemical-resistant gloves, safety glasses with side shields, lab coat
Respiratory Protection: Mask or NIOSH-approved respirator if dust forms
Eye Protection: Safety goggles
Hand Protection: Nitrile gloves
Skin Protection: Protective clothing as required
Exposure Limits: No occupational exposure limits assigned; handle as laboratory chemical
Environmental Controls: Prevent release to environment and working area drains

Physical and Chemical Properties

Appearance: White to off-white powder
Odor: Odorless
pH (1% solution): Around neutral, typically 7.0–7.6
Melting/Freezing Point: Not determined
Boiling Point: Not applicable (solid product)
Solubility: Easily soluble in water
Vapor Pressure: Not applicable
Vapor Density: Not applicable
Relative Density: Not applicable
Partition Coefficient (n-octanol/water): Not determined
Viscosity: Not applicable
Decomposition Temperature: Not established

Stability and Reactivity

Chemical Stability: Stable under ordinary laboratory conditions
Hazardous Reactions: Unlikely under normal use
Conditions to Avoid: Extreme moisture, direct sunlight, temperature extremes
Incompatible Materials: Strong acids, strong bases, oxidizers
Hazardous Decomposition: Thermal breakdown can produce toxic oxides of carbon and nitrogen

Toxicological Information

Likely Routes of Exposure: Skin and eye contact, inhalation
Symptoms: Mild irritation to eyes, skin, or respiratory tract; not known to cause acute health effects
Toxicity Data: Incomplete for mixture as a whole; components at levels used generally regarded as safe for laboratory exposure
Chronic Exposure: Long-term effects not documented at doses used in cell culture
Carcinogenicity: Not listed by IARC, NTP, or OSHA
Mutagenicity/Teratogenicity: No known effects

Ecological Information

Ecotoxicity: No data for mixture; individual ingredients present minimal environmental risk in laboratory quantities
Mobility: Water-soluble components capable of moving in aquatic environment
Bioaccumulation: Not expected
Persistence/Degradability: Expected to degrade and dilute rapidly in water systems at laboratory-scale release
Other Adverse Effects: Avoid large discharges to local environment to minimize unnecessary nutrient load

Disposal Considerations

Waste Disposal Methods: Dispose as laboratory chemical waste; follow all regional, national, and local laws
Container Disposal: Triple rinse containers before disposal or recycling
Waste Handling Precautions: Minimize dust, avoid splashing during liquid disposal

Transport Information

UN Number: Not regulated as hazardous for transport
Proper Shipping Name: Laboratory Chemical Mixture
Transport Hazard Class: Not classified
Packing Group: Not assigned
Environmental Hazards: Not hazardous
Special Precautions for Transport: Protect from moisture and excessive heat during transport; use tightly sealed packaging

Regulatory Information

Labeling: Not hazardous under OSHA Hazard Communication Standard
TSCA: All components listed or exempt
SARA Title III: Not subject to reporting
WHMIS Classification: Not controlled
REACH: Components meet requirements or are exempt as research chemical
Other Regulations: Use consistent with good laboratory practices and all applicable sector guidelines