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Product Name: HRP-Streptavidin
Synonyms: Horseradish Peroxidase-Streptavidin Conjugate
Product Code: Provided by supplier
Supplier: Identified distributor or laboratory supplier
Emergency Contact Number: Listed by organization purchasing or manufacturing
Recommended Use: Laboratory reagent for biomedical and research applications
Restrictions on Use: Not for drug, household, or food use
Classification: Not classified as hazardous under GHS criteria
Label Elements: Pictograms not required; precautionary phrases may include recommendations for safe laboratory handling
Signal Word: None mandated
Hazard Statements: Inhalation, ingestion, or contact can cause mild irritation to skin, eyes, or respiratory tract
Precautionary Statements: Use in a well-ventilated area, avoid skin and eye contact, wash exposed skin after handling
Acute Effects: Potential for skin and eye irritation; possible minor gastrointestinal upset on ingestion
Chemical Name: Streptavidin conjugated to Horseradish Peroxidase
CAS Number: Streptavidin: 9013-20-1; Horseradish Peroxidase: 9003-99-0
Concentration: Solution, typically 0.1–1 mg/mL (refer to product label for exact concentrations)
Formulation: Aqueous buffer with stabilizers and possibly low concentrations of preservatives (e.g., sodium azide below 0.1%)
Impurities: No significant impurities subject to reporting
Additives: Stabilizing proteins and buffer salts characteristic for conjugate stability
Inhalation: Move to fresh air. Seek medical attention if symptoms like coughing or respiratory distress persist.
Skin Contact: Remove contaminated clothing. Rinse skin with water; use soap if available. Seek medical care for irritation.
Eye Contact: Rinse cautiously with water for at least 15 minutes, lifting eyelids. Consult a healthcare provider if irritation or redness remains.
Ingestion: Rinse mouth with water. Do not induce vomiting. Obtain medical advice if abnormal effects persist.
Symptoms and Effects: Usually minor irritation; rare cases of allergic response possible from protein-based reagents.
Suitable Extinguishing Media: Water spray, carbon dioxide, foam, dry chemical, as appropriate for surrounding materials
Unsuitable Extinguishing Media: No special restrictions known
Special Hazards: Organic protein and buffer components may fuel combustion, generating nitrogen oxides, carbon monoxide, carbon dioxide
Firefighter Protection: Use full protective gear and self-contained breathing apparatus
Hazardous Combustion Products: Smoke, toxic vapors like oxides of nitrogen and carbon
Personal Precautions: Wear gloves, protective eyewear, and lab coat. Avoid inhaling vapors or contact with skin and eyes.
Environmental Precautions: Prevent runoff into sewers or water sources; contain spill with absorbent non-combustible material.
Cleanup Methods: Absorb with inert material such as paper towel or diatomaceous earth. Collect in a suitable chemical waste container. Rinse spill area with water and detergent.
Notification Requirements: No specific community notification required unless involved preservative triggers reporting thresholds (e.g., sodium azide).
Handling: Wear recommended protective equipment. Open containers in ventilated areas. Minimize exposure to airborne vapors or splashes.
Storage: Store at specified temperature (commonly 2–8°C, do not freeze unless recommended). Keep containers tightly closed. Separate from food and incompatible chemicals.
Incompatibilities: Strong oxidizers, acids, bases, and agents that degrade proteins.
Hygiene Practices: Wash hands after use; avoid eating, drinking, or smoking in laboratory areas.
Exposure Limits: No established occupational exposure limits for HRP or streptavidin; monitor for any included preservatives.
Engineering Controls: Use in fume hood or ventilated lab bench.
Personal Protection: Lab coat, disposable gloves (nitrile or equivalent), chemical safety goggles.
Respiratory Protection: Not typically required under normal lab use; employ mask if vapors or aerosols are generated.
Environmental Controls: Do not discharge into drains. Collect and treat contaminated waste.
Workplace Monitoring: Monitor procedures involving large volumes or frequent use for exposure risk.
Appearance: Clear or slightly colored aqueous solution
Odor: Faint, characteristic protein solution odor
Odor Threshold: Not determined
pH: 6.0–8.5 (typical for such buffer formulations)
Melting/Freezing Point: Not applicable to solution
Boiling Point: Approximately 100°C (water base)
Flash Point: Not applicable (aqueous, non-flammable)
Evaporation Rate: Similar to water
Flammability: Not flammable
Vapor Pressure: Similar to aqueous solutions
Relative Density: Approximately 1.0 g/mL
Solubility: Fully soluble in water
Partition Coefficient: Not determined
Auto-Ignition Temperature: Not determined
Decomposition Temperature: Above 60°C may denature protein
Viscosity: Low (watery consistency)
Chemical Stability: Stable under recommended storage and handling conditions
Incompatible Materials: Strong acids, bases, oxidizers, heavy metals
Hazardous Decomposition Products: Combustion may yield nitrogen oxides, carbon oxides
Polymerization: No hazard of dangerous polymerization
Conditions to Avoid: Prolonged exposure to heat, elevated temperatures, and strong light which can denature protein
Reactivity: Low reactivity under ambient laboratory conditions
Routes of Exposure: Inhalation, ingestion, dermal, ocular
Acute Toxicity: No known acute toxic effect at laboratory concentrations; possible mild irritation
Chronic Toxicity: No information indicating cumulative or delayed effects
Skin/Eye Irritation: Possible mild irritation on contact; rinse thoroughly if exposure occurs
Respiratory Sensitization: May cause mild respiratory irritation on excessive inhalation
Carcinogenicity: Neither HRP nor streptavidin classified as carcinogenic by major agencies
Developmental/Reproductive Toxicity: No adverse effects known from the components
Target Organ Effects: No organ toxicity expected at laboratory use levels
Environmental Fate: Proteinaceous substances degrade biologically in the environment
Persistence/Degradability: Generally biodegradable
Bioaccumulation Potential: Not expected to bioaccumulate
Aquatic Toxicity: Minimal expected at laboratory volumes; preservatives may pose risk to aquatic life at high concentrations
Soil Mobility: High, due to water solubility
Other Adverse Effects: Avoid large discharges to waste water to prevent impacts from preservatives and buffer salts
Waste Disposal: Collect in clearly labeled chemical waste containers. Avoid disposal in municipal waste or drains untreated.
Contaminated Packaging: Dispose of empty containers as chemical waste. Rinse and decontaminate before recycling if regulations permit.
Special Precautions: Follow all federal, state, and local environmental and hazardous waste regulations. For large spills, consult environmental authorities for guidance.
Recommended Disposal Method: Incineration or chemical treatment through licensed hazardous waste facility
UN Number: Not regulated as hazardous under major transport regulations
Proper Shipping Name: Not regulated
Transport Hazard Class: Not applicable
Packing Group: Not assigned
Marine Pollutant: Not listed
Special Precautions for Transport: Ship in secondary containment with cold pack as required by stability instructions
Additional Information: Review shipping requirements for included preservatives if above regulatory thresholds
US OSHA Status: Not classified as hazardous
TSCA (Toxic Substances Control Act): Not listed for individual proteins
SARA Title III (EPCRA): Not classified under reportable hazardous substances
Europe (REACH/CLP): Exempt or not classified for laboratory-scale use
Canada WHMIS Status: Not controlled
California Proposition 65: Does not contain listed chemicals
Other International Regulations: Not subject to restrictions in major laboratory safety guidelines
Labeling Requirements: Comply with good laboratory practice standards for reagent labeling and documentation
Additional Restrictions: Observe national laboratory regulations for protein reagents, especially if containing additives like sodium azide
