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Product Name: Folic Acid-Folin Ciocalteu Reagent
Synonyms: Vitamin B9-Folin–Ciocalteu Assay Reagent
Recommended Use: Laboratory analysis, nutritional biochemistry, food science research
Manufacturer: Refer to label or supplier documentation for site-specific source
Emergency Phone Number: Local emergency services or manufacturer-specific hotline
Product Code: Unique facility-specific identifier; locate on the packaging
Relevant Identified Uses: Analytical reagent for polyphenol quantitative assay and folic acid quantification
GHS Classification: Eye irritation (Category 2), Skin irritation (Category 2), Acute aquatic toxicity (Category 3)
Signal Word: Warning
Hazard Statements: Causes skin and eye irritation, harmful to aquatic life
Pictograms: Exclamation mark (irritation symbol), Environment symbol
Precautionary Statements: Avoid breathing dust, wash thoroughly after handling, avoid release to environment, wear protective gloves and eye protection
NFPA Ratings: Health: 2, Flammability: 0, Reactivity: 0
Potential Health Effects: Eye contact leads to redness, watery eyes; skin contact brings redness and dryness; inhalation may irritate nose, headache possible
Main Components: Folic Acid: 59-30-3, 100–200 mg/L; Folin-Ciocalteu reagent: mixture of phosphomolybdic-phosphotungstic acid complexes; sodium carbonate; water
Impurities: May contain trace sodium hydroxide, sulfuric acid depending on source
Concentration: Each component varies with supplier standard; pure Folic acid concentration typically ranges between 0.02–0.1% w/v
Chemical Formula (Folic Acid): C19H19N7O6; for Folin-Ciocalteu reagent: proprietary blends, typically not fully disclosed due to formulation but major species identified above
Eye Contact: Immediately flush with plenty of water for at least 15 minutes, keep eyelids apart, seek medical attention for persistent irritation
Skin Contact: Rinse off skin thoroughly with water and soap, remove contaminated clothing, if rush develops consult physician
Inhalation: Move to fresh air, keep breathing comfortable, use oxygen support in severe respiratory distress, seek medical attention
Ingestion: Rinse mouth, do not induce vomiting, drink water, contact poison control center or physician for further guidance
Symptoms of Exposure: Redness, irritation, headache, gastric upset
Suitable Extinguishing Media: Water spray, foam, carbon dioxide, dry chemical
Unsuitable Extinguishing Media: Avoid high-pressure water streams which may spread material
Special Hazards: Thermal decomposition produces irritating fumes and possibly toxic oxides of nitrogen, carbon monoxide, and sulfur compounds
Protective Equipment: Firefighters use self-contained breathing apparatus and protective clothing
Firefighting Procedures: Use water spray to cool unopened containers, avoid inhaling fumes, clear area of non-essential personnel
Explosion Sensitivity: No data indicating explosion risk under normal storage or expected laboratory uses
Personal Precautions: Wear chemical resistant gloves, safety goggles, lab coat, avoid breathing dust
Environmental Precautions: Prevent further leakage or spillage if safe, keep product away from drains and natural water sources
Cleanup Methods: Sweep up using non-sparking tools, ventilate area, place residue in labelled waste container for disposal according to local regulations
Decontamination: Wash contaminated surfaces with water and mild detergent after removal
Waste Handling: Dispose using specialized chemical waste collection; do not flush to sewer
Handling: Use in well-ventilated area, wear appropriate PPE such as gloves and safety glasses, avoid contact with skin and eyes, do not eat or drink while handling
Storage: Store in tightly closed original container in cool, dry, ventilated place away from heat and incompatible materials
Incompatibilities: Strong oxidizers, reducing agents, some metals, acidic or basic substances
Special Requirements: Keep containers properly labelled, protect from physical damage, reduce moisture and UV light exposure
Storage Temperature: Recommended room temperature (15–25°C), do not allow freezing or overheating
Engineering Controls: Use fume hood or local exhaust ventilation for dust or vapor formation, regular air monitoring
Personal Protective Equipment (PPE): Chemical resistant gloves (nitrile, neoprene), splash-proof goggles, lab apron
Respiratory Protection: Use dust masks if airborne particulates are present, higher-level respirators for major spills
Hygiene Measures: Wash hands after handling, remove contaminated clothing, do not touch eyes or mouth
Occupational Exposure Limits: No specific regulatory limits for folic acid or Folin–Ciocalteu reagent, default to general laboratory chemicals guidelines
Appearance: Folic acid: yellowish crystalline powder; Folin–Ciocalteu reagent: clear to dark blue solution, strong acid odor
Odor: Slightly acidic; some sulfurous tones for reagent
Odor Threshold: Not established
pH: Strongly acidic (Reagent pH typically <1), folic acid in water commonly neutral
Melting Point: Folic acid 250°C (decomposes); no melting for aqueous reagent
Boiling Point: Not applicable for most laboratory preparations (solution)
Flash Point: Not flammable
Solubility: Folic acid slightly water-soluble (<10 mg/L), Folin–Ciocalteu reagent mixes readily with water
Density: Reagent typically 1.1–1.2 g/cm³
Partition Coefficient: Not established for mixtures
Evaporation Rate: No data; aqueous, non-volatile
Upper/Lower Flammability: Not applicable
Vapor Pressure: Not applicable
Chemical Stability: Stable under proper storage and normal laboratory conditions
Reactivity: Reacts with strong oxidizers, incompatible with many metals, can release hazardous fumes when heated
Decomposition Products: Nitrogen oxides, carbon monoxide, sulfur oxides on thermal decomposition
Conditions to Avoid: Extreme temperatures, sources of ignition, incompatible chemicals
Incompatibilities: Avoid reducing agents, strong bases, peroxides, concentrated alkali substances
Hazardous Polymerization: Not expected under standard handling
Acute Toxicity: Oral LD50 for folic acid exceeds 1000 mg/kg (rat); Folin–Ciocalteu reagent data less defined
Chronic Effects: Long-term exposure has limited data; repeated skin or eye exposure leads to irritation
Carcinogenicity: Not classified as carcinogenic through NTP, IARC, or OSHA
Mutagenicity: No evidence in standard laboratory studies
Target Organs: Eyes, skin, respiratory tract
Routes of Exposure: Inhalation, dermal, ocular, ingestion
Irritant Effects: Moderate–strong, especially for powder dust or concentrated reagent
Symptoms of Overexposure: Headache, coughing, nausea, dizziness (rare with normal lab use)
Aquatic Toxicity: Harmful to aquatic organisms, particularly in concentrated discharge; Folin–Ciocalteu reagent reported toxic to invertebrates at high concentrations
Persistence and Degradability: Limited data for full mixtures; breakdown expected but may persist under anaerobic conditions
Bioaccumulation: Not significant for folic acid, possible for trace metals in reagent formula
Mobility in Soil: High water solubility for reagent, limited mobility for folic acid
Environmental Precautions: Prevent release into drains, rivers, or soil without treatment
Other Information: Recommended neutralization and containment prior to sewage disposal; waste collection advisable
Waste Treatment Methods: Collect in appropriate sealed chemical waste container, label for hazardous waste
Handling for Disposal: Use gloves and protective eyewear, treat any spills promptly, follow local hazardous waste protocols
Disposal of Packaging: Empty containers may retain residues, triple rinse before disposal, follow container recycling or hazardous waste recommendations
Special Precautions: Do not dump to landfills or water sources
Regulatory Compliance: Comply with federal, regional, and local regulations addressing chemical disposal and reporting
UN Number: Not regulated as dangerous good for transport under most international codes
Transport Hazard Class: Not classified
Packing Group: Not assigned for folic acid or standard lab-scale Folin–Ciocalteu reagent
Marine Pollutant: Not specifically listed, but caution urged for aquatic toxicity
Transport Precautions: Transport upright, secure containers, protect from direct sunlight; avoid stacking heavy loads
Additional Transport Information: Label all outer containers with detailed product identity
Safety, Health, Environmental Regulations: Folic acid is not specifically listed on toxic substances schedules in US/Europe; Folin–Ciocalteu reagent classified as hazardous preparation in regions depending on concentration
OSHA Status: Not a hazardous chemical per 29 CFR 1910.1200 (for pure folic acid), reagent may be regulated due to strong acids
TSCA Inventory: Components listed
REACH Status (EU): Exempt/lab use quantities; refer to supplier
WHMIS (Canada): Controlled product depending on formulation; labelling must match GHS
Other Requirements: Provide SDS documents to all handlers; keep workplace risk assessments updated; emergency action plans should reference chemical hazards
Liability Statement: Manufacturer assumes no legal responsibility for improper handling or unauthorized use
