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Product Name: Earle’s Balanced Salt Solution
Common Names: EBSS, Balanced Salt Solution
Recommended Use: Cell culture, laboratory reagent
Supplier: Typically found through research supply companies and biological reagent distributors
Emergency Contact: Emergency medical services, Poison Control Center, or local facility safety office
Product Code: Varies by supplier
Hazard Classification: Not classified as hazardous under many GHS guidelines for typical laboratory use
Warning Statements: Irritation risk through eye or skin contact, or if swallowed
Signal Words: None typically required
Potential Health Effects: Mild irritation possible; ingestion in large quantities may disrupt electrolyte balance
Environment Hazards: No known significant environmental hazards in small-scale disposal
Precautionary Statements: Use personal protective equipment, avoid direct exposure
Major Ingredients: Sodium chloride, Potassium chloride, Calcium chloride, Magnesium sulfate, Sodium bicarbonate, D-glucose, Phenol red sodium salt (optional indicator), Purified water
Typical Percentages: Sodium chloride ~6800 mg/L, Potassium chloride ~400 mg/L, Calcium chloride ~200 mg/L, Magnesium sulfate ~200 mg/L, Sodium bicarbonate ~2200 mg/L, D-glucose ~1000 mg/L, Phenol Red <10 mg/L
Chemical Abstracts Service Numbers (CAS): Sodium chloride (7647-14-5), Potassium chloride (7447-40-7), Calcium chloride (10043-52-4), Magnesium sulfate (7487-88-9), Sodium bicarbonate (144-55-8), D-glucose (50-99-7), Phenol red (143-74-8), Water (7732-18-5)
Inhalation: Move exposed individual to fresh air; consult medical support if symptoms persist
Skin Contact: Wash affected area under running water, remove contaminated clothing
Eye Contact: Immediately flush eyes with water for several minutes, seek medical advice for irritation
Ingestion: Rinse mouth out, seek medical guidance if larger quantities are ingested or symptoms develop
Special Instructions: Provide medical personnel with product information
Suitable Extinguishing Media: Water spray, dry chemical, foam, carbon dioxide
Unsuitable Extinguishing Media: No restrictions identified for typical solutions
Specific Hazards: Thermal decomposition may produce hazardous fumes like sodium oxides or carbon oxides
Protective Equipment: Firefighters should use appropriate protective gear and self-contained breathing apparatus
Personal Precautions: Wear laboratory coat, gloves, safety goggles
Environmental Precautions: Prevent large volumes from entering drains or water systems
Cleanup Methods: Absorb with inert material (paper towels, cloth), wipe up residue, clean area with water
Disposal: Collect soaked materials in appropriate waste container for chemical disposal
Handling: Avoid inhalation, ingestion, and contact with eyes or skin; follow laboratory safety protocols
Safe Storage: Store at 2–8°C or as specified by manufacturer; protect from direct sunlight
Storage Containers: Keep tightly sealed in original, properly labeled packaging
Incompatible Materials: Strong acids, strong bases, oxidizers
Engineering Controls: Use in well-ventilated area, with local exhaust if possible
Personal Protective Equipment (PPE): Lab coat, safety glasses or goggles, nitrile or latex gloves
Hygiene Measures: Wash hands after handling, remove contaminated clothing promptly
Workplace Exposure Limits: No occupational exposure limits set for ingredients in dilute aqueous solution
Physical State: Clear, colorless to slightly pink (with Phenol red) liquid
Odor: Odorless
pH: Typically ranges from 7.0 to 7.4 at 25°C
Solubility in Water: Complete
Freezing Point: Slightly below 0°C
Boiling Point: Near 100°C
Specific Gravity: Comparable to water
Vapor Pressure: Same as water
Evaporation Rate: Comparable to water
Chemical Stability: Stable under normal laboratory conditions
Conditions to Avoid: Freezing, excessive heat, contamination
Incompatible Materials: Strong acids, strong bases, oxidizing agents
Decomposition Products: Carbon oxides, sodium oxides, toxic fumes if heated to dryness
Polymerization: Does not undergo hazardous polymerization
Acute Effects: May cause temporary irritation to eyes or skin
Chronic Effects: No major chronic hazards identified for intended laboratory use
Routes of Exposure: Inhalation, skin, ingestion, eye contact
LD50/LC50 Values: Components are present at concentrations much lower than toxic levels
Medical Conditions Aggravated: Individuals with electrolyte imbalance or severe burns should avoid ingestion or exposure
Symptoms of Overexposure: Gastrointestinal discomfort, eye or skin redness, no serious systemic toxicity in standard use
Aquatic Toxicity: Low; solution in typical amounts does not harm aquatic environments
Persistence and Degradability: Inorganic salts are persistent; organic components (glucose, phenol red) degrade rapidly
Bioaccumulation Potential: Negligible for all components in dilute solution
Mobility in Soil: Mobile in aqueous environments
Other Adverse Effects: Discharge of large volumes may alter local water chemistry, especially due to salts
Disposal Methods: Dilute solutions may be disposed of via drain, following local regulations; larger amounts require chemical waste collection
Container Disposal: Rinse thoroughly before recycling or disposal
Special Precautions: Do not release larger quantities into natural watercourses
Waste Codes: Not regulated as hazardous waste in many jurisdictions
UN Number: No UN number assigned for standard solutions
Proper Shipping Name: Not regulated for transport
Hazard Class: Not classified
Packing Group: N/A
Marine Pollutant: No
Special Precautions for Transport: Prevent freezing or exposure to extreme temperatures to preserve solution quality
International Inventories: All ingredients are listed or exempt
OSHA Status: Not hazardous under US OSHA Standard
Canada WHMIS Classification: No required classification
US TSCA Status: All ingredients listed or exempt
EU REACH Status: All components pre-registered or exempt
Labeling Requirements: Routine laboratory chemical labeling with content and hazard symbols if indicated by specific additives
