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Product Name: Dulbecco Modified Eagle Medium
Synonyms: DMEM, Dulbecco's Modified Eagle's Medium
CAS Number: Mixture
Recommended Use: Cell culture medium for laboratory use
Manufacturer: Major life sciences suppliers including Sigma-Aldrich, Thermo Fisher, Gibco
Contact Emergency Number: Refer to manufacturer label for local emergency contact
Product Code: Listed on supplier’s label
Prepared by: Health and Safety Office or Product Development Team
GHS Classification: Not classified as hazardous according to OSHA HazCom Standard
Signal Word: None
Hazard Statements: Not expected to pose health risks under normal conditions
Precautionary Statements: Avoid inhalation and direct contact with eyes, skin, or clothing
Pictograms: None required
Most Important Hazards: Dust may be mildly irritating to respiratory tract
Potential Health Effects: May cause slight eye, skin, respiratory irritation on contact or prolonged exposure
Base Medium: DMEM contains a mixture of salts, amino acids, glucose, vitamins
Sodium Chloride (NaCl): 5800 mg/L
Potassium Chloride (KCl): 400 mg/L
Calcium Chloride (CaCl2): 200 mg/L
Magnesium Sulfate (MgSO4): 97.7 mg/L
Sodium Bicarbonate (NaHCO3): 3700 mg/L
Glucose: 1000–4500 mg/L depending on formulation
L-Glutamine: 584 mg/L
Phenol Red: 15 mg/L (may vary, serves as pH indicator)
Essential and Non-Essential Amino Acids: L-Arginine, L-Leucine, L-Threonine, etc.
Vitamins: Biotin, Choline Chloride, Folic Acid, Pantothenic Acid, etc.
Water: Deionized, used to bring components into solution
Other Ingredients: Trace elements, minor buffer compounds
Inhalation: Move individual to fresh air, seek medical attention if irritation or symptoms persist
Skin Contact: Wash thoroughly with soap and water, remove contaminated clothing
Eye Contact: Rinse cautiously with water for several minutes, remove contact lenses if present and easy to do, continue rinsing
Ingestion: Rinse mouth, drink water, seek medical advice if ill effects appear
Symptoms: May include mild irritation, redness, or discomfort at the site of contact
Suitable Extinguishing Media: Water spray, dry chemical powder, carbon dioxide, foam
Special Hazards: Product itself is not flammable, dried residue may burn; hazardous decomposition products may include carbon oxides, nitrogen oxides
Protective Equipment: Self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes
Advice for Firefighters: Avoid breathing combustion gases; use standard fire-fighting procedures for chemicals in laboratory settings
Personal Precautions: Wear appropriate lab coat, gloves, and safety goggles
Environmental Precautions: Prevent spills from large quantities entering drains or waterways
Cleanup Methods: Absorb with inert material such as sand or vermiculite, sweep up carefully, transfer to closed containers for disposal according to regulations
Decontamination: Wash spill site with water after material pickup is complete to prevent residue accumulation
Precautions for Safe Handling: Avoid inhalation, ingestion, and contact with eyes and skin; wash hands after handling
Safe Storage Conditions: Store at 2–8°C, protect from light to maintain product stability
Storage Materials: Keep in original tightly closed container, do not use damaged packaging
Specific Uses: Cell culture application only—do not use for human or veterinary clinical procedures
Incompatibilities: Strong oxidizers, acids, alkalis—handle away from incompatible materials
Engineering Controls: Laboratory fume hood or well-ventilated area recommended for powder handling
Respiratory Protection: Not required for solution; if generating dust, use NIOSH-approved particulate respirator
Hand Protection: Dispose gloves recommended—nitrile or latex
Eye and Face Protection: Safety glasses with side shields or goggles
Skin Protection: Lab coat, apron, closed shoes, long sleeves
Hygiene Measures: Do not eat, drink, or smoke during handling; wash thoroughly after use
Physical State: Powder or ready-to-use liquid
Color: Yellow-orange to red (due to phenol red indicator)
Odor: Faint, none to mild chemical smell
pH: 7.2–7.4
Boiling Point: Not applicable for powder; for solution, approximately 100°C
Melting Point: Not determined
Solubility in Water: Complete (for powder)
Viscosity: Similar to water for liquid
Flash Point: Not applicable
Evaporation Rate: Not established
Vapor Pressure: Not established
Partition Coefficient: Not established
Decomposition Temperature: Not determined
Autoignition Temperature: Does not ignite easily
Density: Close to water (for liquid)
Chemical Stability: Stable under recommended storage and handling conditions
Reactivity: Non-reactive under standard conditions
Possibility of Hazardous Reactions: Does not undergo hazardous reactions under normal use
Conditions to Avoid: Excessive heat, freezing, direct sunlight
Incompatible Materials: Strong oxidizing agents, acids or bases
Hazardous Decomposition Products: Carbon oxides, nitrogen oxides, possibly sulfur oxides when heated to decomposition
Acute Toxicity: Data not available for the mixture; individual ingredients at listed concentrations are not acutely toxic
Skin Corrosion/Irritation: Not irritating under normal laboratory use
Serious Eye Damage/Irritation: May cause mild irritation
Respiratory Sensitization: No data in the literature; unlikely at concentrations handled in laboratories
Skin Sensitization: Not reported
Mutagenicity: No evidence found for mutagenic effects
Carcinogenicity: No evidence for cancer risk, mixture not listed by IARC, NTP, OSHA
Reproductive Toxicity: No known effects
Specific Target Organ Toxicity: Not considered a risk at laboratory concentrations
Experience: Decades of use worldwide; exposure risks mainly linked to improper handling and accidental ingestion
Ecotoxicity: Not identified as harmful in the environment at laboratory scale
Aquatic Toxicity: Ingredients present at low concentrations, not expected to be hazardous
Persistence and Degradability: Components are biodegradable
Bioaccumulation Potential: Not likely at concentrations found in DMEM
Mobility in Soil: High solubility increases mobility, but environmental impact insignificant for typical volumes
Other Adverse Effects: No adverse ecological effects reported in published information
Waste Disposal Methods: Dispose of in accordance with local, regional, national, and international regulations
Disposal of Packaging: Rinse containers, recycle or dispose based on local requirements
Contaminated Packaging: Handle as laboratory waste, incinerate or landfill as permitted
Special Precautions: Prevent release into sewage, drains, or natural waterways
UN Number: No hazardous designation for transport
Transport Hazard Class: Non-hazardous
Packing Group: Not applicable
Proper Shipping Name: Not regulated
Maritime Transport: Not regulated as a marine pollutant
Special Precautions for User: Avoid direct sunlight and extreme temperatures during transport
International Air Transport Association (IATA): Not regulated
Department of Transportation (DOT): Not regulated
OSHA Status: Not hazardous under OSHA Hazard Communication Standard
TSCA Inventory: Some ingredients listed, most as mixtures for research use
SARA Title III Section 313: Not subject to reporting
California Proposition 65: Contains no substances known to the state of California to cause cancer or reproductive toxicity
Canadian WHMIS Classification: Not regulated
REACH (Europe): Exempt for research and development
International Inventories: Components are widely accepted for laboratory use, not subject to additional labeling requirements
Specific Regulations: Follow institutional biosafety and chemical hygiene procedures at all times
