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Product Name: Dimidium Bromide
Chemical Formula: C21H20BrN3
Synonyms: Ethidium Bromide Homologue, 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Recommended Use: Laboratory reagent, nucleic acid stain
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CAS Number: 518-67-2
Hazard Classification: Harmful if swallowed, suspected mutagen, reproductive toxicity suspicion, skin and eye irritant
Signal Word: Warning
Hazard Statements: May cause genetic defects, suspected of damaging fertility or the unborn child, harmful in contact with skin or if inhaled
Pictograms: Health hazard, exclamation mark
Routes of Exposure: Inhalation, ingestion, skin contact, eye contact
Possible Effects: DNA binding properties pose risk of mutagenesis and carcinogenesis
Component: Dimidium Bromide
Concentration: 98–100%
Molecular Weight: 394.31 g/mol
Impurities: Possible trace organic contaminants, less than 1% not expected to present increased hazard
Ingredient List: Dimidium bromide, inert residuals
General Advice: Seek immediate medical attention if symptoms develop or in the event of exposure to large quantities
Inhalation: Remove person to fresh air, seek medical attention if discomfort persists
Skin Contact: Wash exposed skin with soap and plenty of water for at least 15 minutes, remove contaminated clothing
Eye Contact: Rinse eyes thoroughly with water for at least 15 minutes, lift upper and lower eyelids occasionally, consult a physician
Ingestion: Rinse mouth, do not induce vomiting, seek medical attention at once
Suitable Extinguishing Media: Use dry chemical powder, carbon dioxide, foam, or water spray
Unsuitable Media: High-pressure water jet can spread contamination
Special Hazards: Combustion may produce toxic gases including nitrogen oxides, hydrogen bromide, carbon monoxide; dust can form explosive mixtures
Protective Equipment: Firefighters should wear self-contained breathing apparatus and protective clothing
Additional Advice: Remove containers from area if possible, prevent run-off water entering drains
Personal Precautions: Wear protective gear including gloves, lab coat, eye protection, and mask
Environmental Precautions: Prevent further leakage or spillage if safe, avoid release to environment, confine spill
Containment: Absorb spilled material with inert absorbent (sand, earth), do not use sawdust
Cleanup Methods: Collect spilled material in suitable closed containers, ventilate area, wash site thoroughly after material pickup
Waste Disposal: Send to licensed chemical waste disposal facility
Precautions for Safe Handling: Work in well-ventilated area, avoid contact with skin and eyes, do not breathe dust or vapors, keep away from incompatible materials
Hygiene Measures: Wash hands after handling, remove contaminated clothing, avoid eating, drinking or smoking in work areas
Storage Conditions: Store tightly closed in cool, dry, well-ventilated area, protected from light and incompatible materials
Storage Temperature: Below 25°C
Incompatible Materials: Strong oxidizers, strong acids, light-sensitive compounds
Exposure Limits: No specific occupational exposure limits established
Engineering Controls: Use chemical fume hood, safety shower, eyewash stations
Personal Protective Equipment: Chemical-resistant gloves (nitrile recommended), safety goggles or face shield, lab coat, respiratory protection if dust/vapors possible
Environmental Controls: Minimize release to air, prevent material entering drains and waterways, handle only by trained personnel
Appearance: Orange-red crystalline powder
Odor: Odorless
Melting Point: 180–184°C
Boiling Point: Decomposes before boiling
Solubility: Soluble in water, moderate solubility in ethanol
pH: Not applicable in solid form, typically neutral in solution
Vapor Pressure: Negligible at room temperature
Density: 1.3–1.5 g/cm³
Flash Point: Not applicable with typical use
Other Properties: Stains skin and materials persistently
Chemical Stability: Stable under recommended storage and handling conditions, unstable in presence of strong oxidizers
Conditions to Avoid: Light, heat, strong acids, and bases
Hazardous Reactions: May react with strong oxidizing agents and reducing agents, decomposition releases toxic gases
Hazardous Decomposition Products: Hydrogen bromide, nitrogen oxides, carbon monoxide, carbon dioxide
Polymerization: Does not occur under normal conditions
Acute Toxicity: May have moderate oral and dermal toxicity, inhalation studies lacking but possible respiratory irritation suspected
Chronic Effects: Evidence from DNA intercalating activity suggests possible mutagenic, teratogenic, and carcinogenic effects
Routes of Exposure: Skin, eye, inhalation, ingestion
Symptoms: Skin irritation, redness, eye irritation, gastrointestinal discomfort, possible mutagenic risks with prolonged exposure
LD50 (rat, oral): Estimated to be 300–500 mg/kg
Other Data: Prolonged and repeated exposure presents increased risk of genetic damage
Toxicity: Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment
Persistence and Degradability: Not readily biodegradable; may persist in soil and water
Bioaccumulative Potential: Limited information, persistence suggests risk
Mobility in Soil: Likely to leach into soil given water solubility
Other Adverse Effects: Hazard to ecosystem, avoid release into environment
Waste Treatment Methods: Handle as hazardous laboratory waste
Disposal of Product: Collect and dispose through approved chemical waste vendor
Decontamination: Contaminated containers and packaging treated as hazardous, avoid landfill; triple rinse and render packaging unusable
Special Precautions: Do not allow to reach sewage system, ground water, or surface water
UN Number: Not assigned for common quantities in laboratory use
Proper Shipping Name: Laboratory chemical, N.O.S. (Dimidium Bromide)
Transport Hazard Class: Toxic, possible environmental hazard
Packing Group: III
Labels: Toxic substance, environmental hazard if shipped in large quantity
Other Requirements: Secure packaging to prevent leaks, transport in compliance with local and international ADR, IMDG, IATA rules
Emergency Information: Provide MSDS during transport for first responders
Labelling Requirements: Based on laboratory chemical hazard, requires clear label with chemical name, concentration, and hazard pictograms
Regulations: Subject to OSHA laboratory standard, EU REACH regulation, TSCA inventory for the United States, and local environmental protection acts
Workplace Classification: Harmful, mutagenic substance by local regulations
EH&S Program: Routine training and monitoring for personnel handling material
Other Restrictions: Not approved for pharmaceutical or food use—laboratory use only
