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Product Name: Cell Freezing Medium, DMSO Serum Free
Product Use: Preserves and protects cells during freezing
Manufacturer: Biotech supplier; see package label for details
Emergency Telephone: Poison Control Center, local emergency numbers
Synonyms: Cryopreservation Medium, DMSO Cryosolution
Catalog Numbers: Refer to packaging for precise code
Date Revised: Refer to current shipment or supplier
GHS Classification: Eye irritation (Category 2A), Skin irritation (Category 2), Specific Target Organ Toxicity (STOT) single exposure (Category 3) for DMSO
Hazard Statements: Causes serious eye and skin irritation; may cause respiratory irritation
Precautionary Statements: Avoid breathing vapors, wear eye/face protection, wash hands thoroughly after handling
Signal Word: Warning
Pictograms: Exclamation mark
Routes of Exposure: Skin, inhalation, ingestion, eye contact
Symptoms: Redness, itching, watering of eyes, skin discomfort, throat irritation, headache
DMSO (Dimethyl Sulfoxide): 7.5-10%
Proprietary Additives: Polysaccharide stabilizers, osmoprotectants, small molecule cryoprotectants
Saline Base: Balanced salt solution for isotonicity
CAS Number (DMSO): 67-68-5
Impurities: No hazardous impurities present above threshold limits
Serum-free Guarantees: No animal-derived components
Full ingredient disclosure: Provided by supplier upon request for regulatory or clinical needs
Inhalation: Move to fresh air, seek medical attention if symptoms persist
Skin Contact: Wash thoroughly with soap and water; remove contaminated clothing
Eye Contact: Rinse cautiously with water for several minutes; remove contact lenses if present
Ingestion: Do not induce vomiting, rinse mouth and call physician or Poison Control
Medical Advice: Treat symptomatically; DMSO rapidly crosses skin and mucous membranes
Suitable Extinguishing Media: Water spray, dry chemical, carbon dioxide, foam
Unsuitable Extinguishing Media: High pressure water jets may spread fire
Special Hazards: DMSO can produce toxic gases under fire (sulfur oxides, formaldehyde)
Firefighter Protection: Self-contained breathing apparatus, chemical resistant clothing
Explosion Risk: Containers may rupture under extreme heat; no explosive properties under normal use
Spill Clean-Up: Absorb with inert material, use non-sparking tools, collect in a sealed waste container
Personal Precautions: Wear gloves, lab coat, eye/face protection
Ventilation: Ensure adequate ventilation, evacuate area for significant spills
Environmental Precautions: Prevent from entering drains, surface waters, soil
Decontamination Procedures: Wash spill area with water after removal
Storage: Store at -20°C or colder for long-term; avoid repeated freeze-thaw cycles
Handling Conditions: Avoid direct contact and inhalation of vapors; keep containers tightly closed
Incompatibilities: Store away from strong oxidizers, acids, alkalis
Hygiene Practices: Wash hands before breaks and after handling; do not eat, drink, or smoke
Storage Area: Use dedicated cryogenic or laboratory freezer; keep labeled and segregated from incompatible materials
Occupational Exposure Limits: No established limits for DMSO, minimize exposure as a precaution
Engineering Controls: Use in well-ventilated area, fume hood recommended for large volumes
Personal Protective Equipment (PPE): Nitrile or latex gloves, lab coat, chemical splash goggles, risk of splash calls for face shield
Respiratory Protection: Not typically required in normal lab use; use a mask if vapor exceeds nuisance levels
Other Controls: Emergency eyewash stations and showers should be accessible
Appearance: Clear, colorless to pale yellow solution
Odor: Mild characteristic odor (DMSO)
pH: 7.0–8.0
Boiling Point: Above 100°C due to aqueous components
Melting/Freezing Point: Below 0°C; varies with composition
Flash Point: >87°C for DMSO
Vapor Pressure: Low at room temperature
Solubility: Completely soluble in water
Density: 1.02–1.05 g/cm³
Viscosity: Slightly more viscous than water
Partition Coefficient: Not applicable; designed to remain in solution
Chemical Stability: Stable under recommended storage conditions
Thermal Decomposition: DMSO degrades at high temperatures, producing hazardous gases
Reactivity: Reactive toward strong oxidizers and some organic materials
Hazardous Reactions: Avoid contact with acids, strong reducing agents
Polymerization: Will not occur
Storage Incompatibilities: Metals, concentrated acids, bases, oxidizers
Acute Toxicity: Ingestion or injection may cause nausea, headache, dizziness, skin irritation
Chronic Toxicity: Prolonged exposure to DMSO may impact liver and kidney function in animal studies
Irritation: DMSO penetrates skin and may cause transient garlic-like taste, discomfort
Carcinogenicity: Not classified as carcinogenic by IARC, NTP, OSHA
Reproductive Effects: No known reproductive risks in humans under normal laboratory use
Sensitization: No data indicating respiratory or skin sensitization at typical laboratory exposures
Aquatic Toxicity: Dilute releases are not expected to harm aquatic organisms; high concentrations may be harmful to aquatic invertebrates
Persistence and Degradability: DMSO is biodegradable, expected to degrade rapidly in industrial wastewater systems
Bioaccumulation: Does not bioaccumulate specifically; passes through organisms rapidly
Mobility in Soil: High mobility due to water solubility
Other Effects: Avoid release to surface waters, use proper lab procedures to limit exposure
Waste Treatment Methods: Treat as laboratory chemical waste; follow institutional and local hazardous waste disposal regulations
Disposal Containers: Use labeled, sealed containers for disposal
Avoid: Flushing large quantities down drains
Empty Container Handling: Triple rinse containers prior to recycling or disposal; deface labels
Environmental Precautions: Do not release to environment; document all waste removal activities
UN Number: Not regulated under most transport regulations due to aqueous, dilute formulation
Proper Shipping Name: Non-regulated medical reagent
Hazard Class: Not classified as hazardous
Packing Group: Not applicable
Special Precautions: Maintain cold chain shipping to avoid product degradation; protect containers from rupture due to freezing
DOT / IATA / IMDG: Not restricted in commerce by air, sea, or road at laboratory volumes
OSHA: Not hazardous under US OSHA hazard communication standard
SARA Title III: Not listed as a reportable hazardous material
TSCA Status: DMSO listed on TSCA inventory
REACH (EU): Components pre-registered or exempt
WHMIS (Canada): Not controlled product
Other Regulations: Not subject to California Proposition 65, not regulated under GHS for most end uses
Labeling: Laboratory use only, not for drug, household, or food application
