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Product Name: 30% Acrylamide-Bisacrylamide Solution
Common Names: Acrylamide:bisacrylamide solution, premixed gel solution
Product Use: Laboratory gel preparation, electrophoresis
Manufacturer: Listed by laboratory supply companies
Contact Information: Available through supplier or safety officer
Emergency Telephone: Listed on supplier’s information or local poison control
Synonyms: Acryl-Bis mix, Acrylamide stock
CAS Numbers: Acrylamide (79-06-1), N,N’-Methylenebisacrylamide (110-26-9)
UN Number: Not assigned for solutions of these concentrations
GHS Classification: Acute toxicity (oral, dermal), skin sensitizer, neurotoxin
Signal Word: Danger
Hazard Statements: Harmful if swallowed. Suspected of causing cancer. May cause damage to nervous system. Causes skin and eye irritation. Toxic to aquatic life.
Pictograms: Skull and crossbones, exclamation mark, health hazard
Routes of Exposure: Inhalation, skin/eye contact, ingestion
Potential Health Effects: Tingling in hands and feet, dizziness, muscle weakness, eye redness, skin rash
Long-Term Risks: Probable carcinogen, neurological disorders, reproductive harm
NFPA Ratings: Health 3, Fire 1, Reactivity 1
Acrylamide: 29% by weight; CAS 79-06-1
N,N’-Methylenebisacrylamide: 1% by weight; CAS 110-26-9
Water: 70% by weight
Impurities: None identified above 0.1% w/w
Stabilizers: May contain trace inhibitors to prevent polymerization during storage
Mixture Description: Liquid, colorless solution with no visible particles
Threshold Limits: Acrylamide ACGIH TLV 0.03 mg/m3 (skin)
Eye Contact: Rinse cautiously with water for several minutes. Remove contact lenses. Seek medical attention if redness or pain persists.
Skin Contact: Remove contaminated clothing. Wash affected skin with soap and plenty of water. Call a physician for persistent irritation.
Inhalation: Move person to fresh air. Monitor for signs of neurotoxicity such as numbness or headache. Administer artificial respiration if breathing has stopped.
Ingestion: Rinse mouth thoroughly. Do not induce vomiting. Give small amounts of water if conscious. Seek immediate medical help.
Special Notes: Symptoms may be delayed. Medical monitoring for exposed personnel recommended.
Suitable Extinguishing Media: Water spray, carbon dioxide, dry chemical, alcohol-resistant foam
Unsuitable Extinguishing Media: Do not use direct water jet as solution may splash.
Specific Fire Hazards: Decomposes under intense heat to form toxic gases including nitrogen oxides, carbon monoxide, carbon dioxide
Special Firefighting Procedures: Use self-contained breathing apparatus and full protective clothing
Thermal Decomposition Products: Acrylamide monomer, bisacrylamide, ammonia, carbon oxides
Flammability Class: Not classified as flammable, but solution may support combustion of other materials
Personal Precautions: Wear nitrile gloves, lab coat, safety goggles. Avoid inhalation and skin contact.
Spill Containment: Remove ignition sources. Stop leak if safe to do so. Use non-sparking tools.
Cleanup Procedure: Absorb with inert material (sand, vermiculite). Collect liquid in a sealable chemical waste container.
Decontamination: Clean spill area thoroughly with water and detergent.
Waste Disposal: Label and dispose with hazardous chemical waste.
Environmental Precautions: Prevent entry into drains, surface water, soil.
Handling: Work in chemical fume hood. Minimize aerosol and vapor generation. Wash hands after use.
Storage Conditions: Store locked up, in cool, dry, well-ventilated area, away from incompatibles.
Incompatibilities: Strong acids, strong bases, oxidizing agents, reducing agents, heavy metals (may catalyze polymerization)
Packaging Materials: Use only original, tightly capped chemically-resistant containers.
Special Precautions: Inspect containers regularly for leaks or crystallization.
Engineering Controls: Use local exhaust ventilation, certified chemical fume hood.
Personal Protective Equipment: Chemical-resistant gloves (nitrile, not latex), safety goggles or face shield, impermeable lab coat.
Respiratory Protection: Use NIOSH/MSHA-approved respirator if vapor or aerosol concentrations exceed limits.
Hygiene Measures: Do not eat, drink, or smoke in areas where acrylamide is handled. Wash hands and face thoroughly after handling.
Exposure Limits: Acrylamide ACGIH TLV 0.03 mg/m3 (as inhalable fraction, skin notation)
Appearance: Clear, colorless liquid
Odor: Faint characteristic odor, often described as amine-like
Odor threshold: Low, not a reliable indicator of exposure
pH: Near neutral (6–7.5)
Melting/Freezing Point: <0 °C
Boiling Point: 100 – 102 °C
Flash Point: Not applicable
Evaporation Rate: Similar to water
Flammability: Not classified as flammable
Vapor Pressure: Low, similar to water
Solubility: Miscible with water in all proportions
Density: 1.03–1.05 g/mL at 20°C
Viscosity: Slightly higher than water
Partition Coefficient: Log Kow -0.67 (acrylamide monomer)
Decomposition Temperature: >120°C
Chemical Stability: Stable in unopened containers at room temperature;
Polymerization: Risk increases with heat, light, and contamination. Free radical initiators trigger rapid solidification.
Hazardous Reactions: Exothermic polymerization may produce heat and pressure.
Incompatible Materials: Strong acids, strong bases, oxidizers, heavy metal salts, reducing agents
Hazardous Decomposition Products: Ammonia, carbon monoxide, carbon dioxide, nitrogen oxides
Routes of Exposure: Inhalation, ingestion, skin/eye absorption
Acute Toxicity: LD50 (oral, rat) 124 mg/kg for acrylamide; bisacrylamide similar
Chronic Effects: Nervous system damage, peripheral neuropathy, muscle weakness
Carcinogenicity: Listed as likely human carcinogen by IARC (Group 2A), NTP
Mutagenicity: Positive results in bacteria and mammalian cells
Reproductive Toxicity: Evidence of reproductive toxicity (animal studies)
Developmental Toxicity: Developmental effects observed in animal data
Other Effects: Probable cumulative toxicity with repeated exposure
Ecotoxicity: High toxicity to aquatic invertebrates, fish, algae
Persistence and Degradability: Acrylamide is biodegradable under certain conditions, but may persist in groundwater
Bioaccumulation Potential: Low log Kow; not likely to bioaccumulate
Mobility in Soil: High mobility; may contaminate groundwater
Aquatic Toxicity: LC50 (fish, 96 hr): 136 mg/L acrylamide
Additional Information: Avoid discharge to sewers, waterways, soil
Waste Disposal Method: Dispose as hazardous chemical waste in accordance with federal, state, local regulations
Contaminated Packaging: Rinse and dispose according to hazardous waste guidelines
Incineration: Permitted in licensed, regulated chemical incinerators
Precautions: Do not mix with regular trash or pour into drains
Recommended Waste Codes: US EPA D003 (reactive waste), may also qualify as D001 or D043 depending on state regulations
UN Number: Not regulated under United Nations recommendations for this concentration
DOT Classification: Not classified as dangerous for transport in diluted aqueous solution
Transport Hazard Class: None assigned at 30% concentration
Packing Group: Not assigned
Special Precautions: Secure bottles upright, label “Toxic, handle with gloves, prevent breakage”
Emergency Response Guide: Refer to transport documentation for specific accidental release plans
OSHA: Acrylamide monomer subject to workplace reporting (29 CFR 1910.1200)
SARA Title III: Acrylamide listed as hazardous under Sections 302, 313
TSCA Inventory: Acrylamide and bisacrylamide are on the inventory
California Proposition 65: Acrylamide listed for cancer and developmental toxicity
WHMIS (Canada): D1A (Very toxic, acute), D2A (Carcinogen, chronic hazard)
REACH (EU): Substance of Very High Concern (SVHC) – acrylamide
Label Requirements: Toxic, suspected carcinogen, handle using strict laboratory hygiene
