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Product Name: 2,2'-Azino-Bis(3-ethylbenzothiazoline-6-sulfonic Acid)
Synonyms: ABTS, ABTS Diammonium Salt
Chemical Formula: C18H18N4O6S4
CAS Number: 30931-67-0
Recommended Use: Colorimetric substrate in enzymatic assays, particularly for peroxidase enzymes like horseradish peroxidase in research laboratories
Supplier Details: Contact chemical provider directly for emergency contact numbers and support information
Emergency Contact: Refer to local regulations for chemical emergency responses
GHS Classification: Irritant to skin, eyes, and respiratory tract; not classified as carcinogenic, mutagenic, or reprotoxic based on available data
Signal Word: Warning
Hazard Statements: Causes mild skin and eye irritation; may cause respiratory tract irritation; precautionary measures suggested for long-term repeated exposure
Pictograms: Exclamation mark (Irritant)
Precautionary Statements: Avoid breathing dust; wash thoroughly after handling; use personal protective equipment; handle in a well-ventilated area
Long-Term Effects: Extended exposure may increase risk of allergic skin reactions or minor sensitization
Chemical Name: 2,2'-Azino-Bis(3-ethylbenzothiazoline-6-sulfonic Acid)
Concentration: 98-100% (for pure form, check for diluents or stabilizers with supplier)
CAS Registry Number: 30931-67-0
Molecular Weight: 548.65 g/mol
Impurities: No hazardous impurities at levels relevant for human health under normal laboratory use
Inhalation: Move individual outdoors to fresh air; seek medical attention if persistent discomfort or symptoms such as coughing, shortness of breath, or irritation occur
Skin Contact: Wash affected area with ample soap and water; remove contaminated clothing; seek medical advice if irritation develops
Eye Contact: Flush eyes immediately with running water for at least 15 minutes, keeping eyelids apart; seek medical evaluation if irritation or redness remains
Ingestion: Rinse mouth with plenty of water; avoid inducing vomiting; obtain medical attention if feeling unwell or after large ingestion
Symptoms: Irritation of respiratory passages, skin rash, eye redness, mild gastrointestinal discomfort
Suitable Extinguishing Media: Water spray, dry chemical, foam, or carbon dioxide
Unsuitable Extinguishing Media: None known for this material based on laboratory settings
Specific Hazards: May produce sulfur oxides, nitrogen oxides, and carbon oxides on decomposition; smoke and combustion byproducts can irritate
Protective Equipment for Firefighters: Wear self-contained breathing apparatus and protective clothing to prevent exposure
Advice for Firefighters: Use caution with run-off water; contain and collect contaminated extinguishing water to prevent environmental release
Personal Precautions: Use gloves, lab coat, and chemical splash goggles; avoid dust generation and inhalation
Emergency Procedures: Ensure adequate ventilation; evacuate unnecessary personnel from spill area
Environmental Precautions: Do not allow product to reach soil, waterways, or drainage systems; prevent bulk release outside containment area
Methods for Containment and Cleanup: Sweep up solid material carefully and transfer to closed, labeled waste container; wash residual area with water; dispose of in compliance with local regulations
Handling: Avoid contact with skin, eyes, and clothing; handle in fume hood if dust production likely; wear appropriate PPE including nitrile gloves and safety goggles at all times; do not eat, drink, or smoke in workspace
Storage: Store in tightly closed original container in a dry, cool, well-ventilated area; keep away from oxidizing agents and strong acids; store below 25°C; protect from direct sunlight and moisture; keep out of reach from unauthorized personnel
Control Parameters: No specific regulatory occupational exposure limits established for this compound
Engineering Controls: Use local exhaust ventilation or a certified chemical fume hood to minimize airborne concentrations
Personal Protective Equipment (PPE): Lab coat, chemical resistant gloves, safety goggles or face shield; consider a respirator if dust formation is significant and ventilation is insufficient
Hygiene Measures: Wash hands thoroughly after handling; avoid contact with face; remove contaminated clothing and wash before reuse; routine environmental monitoring rarely necessary unless exposed frequently
Appearance: Deep green to blue-green crystalline powder or solid
Odor: Odorless
Odor Threshold: Not determined
pH: 3.2 – 4.0 in aqueous solution (10g/L)
Melting Point/Range: >250°C (decomposes without melting)
Boiling Point: Not applicable for this solid
Solubility: Freely soluble in water; limited solubility in ethanol; insoluble in most organic solvents
Vapor Pressure: Negligible at room temperature
Vapor Density: Not applicable
Density: Not determined
Partition Coefficient (n-octanol/water): Not available
Decomposition Temperature: Above 250°C; releases toxic gases
Flash Point: Not relevant; non-flammable
Flammability: Not classified as flammable
Explosive Properties: Low risk
Chemical Stability: Stable under recommended storage conditions for laboratory chemicals
Reactivity: Reacts with strong oxidizers, reducing agents, and strong acids; may discolor or degrade upon prolonged exposure to light, heat, or moisture
Hazardous Decomposition Products: Sulfur oxides, nitrogen oxides, carbon monoxide and carbon dioxide
Conditions to Avoid: Strong heat, direct sunlight, incompatible materials, and humid conditions; risk of slow decomposition in presence of strong acids and bases
Polymerization: No hazardous polymerization expected under standard conditions
Acute Toxicity: Low toxicity expected based on animal studies and extensive research use
Routes of Exposure: Inhalation, ingestion, skin contact, eye contact
Symptoms: Irritation of respiratory tract, skin, and mucous membranes; redness and discomfort in eyes; no evidence of chronic toxicity or carcinogenicity reported
LD50/LC50: Not defined for humans or common test animals in public literature
Chronic Effects: Extended or repeated handling can cause mild sensitization; extensive use in laboratories with standard PPE rarely leads to lasting harm
Carcinogenicity, Mutagenicity, Reproductive Toxicity: No data to show significant hazards in these areas
Other Data: Not listed as hazardous by OSHA, IARC, NTP, or EU restrictions
Ecotoxicity: Research suggests limited acute toxicity to aquatic organisms at laboratory-use concentrations, but broader environmental impact has not been fully evaluated
Persistence and Degradability: Likely to degrade in the environment; degradation products require further study
Bioaccumulative Potential: Expected to have low bioaccumulation potential based on chemical structure
Mobility in Soil: Moderate mobility if spilled; soluble in water, can move through soil to some degree
Other Adverse Effects: Avoid bulk release to water bodies due to risk to aquatic life from concentrated solutions
Waste Treatment Methods: Mix residual product with suitable absorbent material and dispose of in accordance with national and regional chemical waste regulations
Container Disposal: Completely empty containers before final disposal; rinse and discard according to institutional protocol for hazardous waste
Special Precautions: Do not allow chemical to drain into water systems, soil, or access points to sewage networks
Incineration: Permissible if approved by local authorities; avoid uncontrolled burning
Reuse/Recycle: Not advisable due to contamination risks
UN Number: None assigned for this product as it is not regulated as a dangerous good for transport
Proper Shipping Name: Not regulated for transport by air, sea, or road under global shipping standards
Transport Hazard Class: Not classified
Packing Group: Not applicable
Environmental Hazards: Not classified as hazardous to the environment in transport
Special Transport Precautions: Keep container sealed and upright during transport; avoid extreme temperatures and physical damage
US Regulations: Not listed on the EPA Toxic Substances Control Act (TSCA) inventory for industrial use; controlled under standard OSHA laboratory safety guidelines
Europe: Not subject to REACH registration as a research chemical in small quantities; follows safety data sheet (SDS) and workplace chemical safety rules
Canada: Meets workplace hazardous materials regulations; not listed under WHMIS as a controlled product
Global: Safe use, handling, and disposal must comply with local country health and safety rules; check institutional policy for new findings
Label Requirements: Chemical hazard information must be clear on all labels: includes product name, hazard pictogram, signal word, and emergency instructions
